You are here:
GENERATION OF RECOMBINANT BACULOVIRUS VIA LIPOSOME MEDIATED TRANSFECTION
Citation:
Hartig, P., M. Cardon, AND C. Kawanishi. GENERATION OF RECOMBINANT BACULOVIRUS VIA LIPOSOME MEDIATED TRANSFECTION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/025 (NTIS PB92143973).
Description:
Baculovirus expression vectors have become a popular method of producing recombinant proteins. Production of recombinant virus requires the transfection of both the native viral DNA and a transfer plasmid into insect cells where recombination takes place. While several methods of transfecting insect cells exist, we have found liposome mediated transfection to be the most efficient. Here we detail the protocols and medium needed for efficient simple transfection of Spodoptera frugiperda cells.
Record Details:
Record Type:DOCUMENT( REPORT )
Product Published Date:05/24/2002
Record Last Revised:04/16/2004
Record ID:
30138