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EFFECT OF AMPICILLIN-INDUCED ALTERATIONS IN MURINE INTESTINAL MICROBIOTA ON THE SURVIVAL AND COMPETITION OF ENVIRONMENTALLY RELEASED PSEUDOMONADS
Citation:
George, S., D. Walsh, A. Stead, AND L. Claxton. EFFECT OF AMPICILLIN-INDUCED ALTERATIONS IN MURINE INTESTINAL MICROBIOTA ON THE SURVIVAL AND COMPETITION OF ENVIRONMENTALLY RELEASED PSEUDOMONADS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/442 (NTIS PB91109348).
Description:
The environmental release of genetically altered microorganisms has prompted the investigation of their potential health effects by employing in vivo rodent models. ven though the direct health effects are addressed, the investigation primarily examines some potential indirect health effects. he model was used previously to examine several mutant Psuedomonas species from a commercial product for polychlorinated biphenyl (PCB) biodegradation. ndirect effects examined include the colonization of the gastrointestinal tract, competition with the resident microbiota, and translocation of the dosed microorganisms to other organs. mpicillin treatment of the mice enhanced the survival of the PCB degraders. hen dosed by gavage with approximately 10 9 of the indicated ampicillin resistant pseudomonad, up to a three-fold increase in recovery was observed 48 hours after dosing. mpicillin treatment also had a significant effect on the microbiota in the intestine, by selecting primarily for the facultative species. he alteration of the lactose-fermenting enterobacteria, obligately anaerobic predominantly Gram-negative rods, and total aerobic and anaerobic populations were monitored in the presence and absence of the PCB-degrading pseudomonad. epending on the dosed strain, a significant difference in the monitored populations as compared to control animals was observed. o translocation of the dosed strains to the liver or spleen was observed 48 hours after dosing.