You are here:
UPTAKE OF INORGANIC LEAD IN VITRO BY ISOLATED MITOCHONDRIA AND TISSUE SLICES OF RAT RENAL CORTEX
Citation:
Kapoor, S., G. Rossum, K. O'Neill, AND I. Mercorella. UPTAKE OF INORGANIC LEAD IN VITRO BY ISOLATED MITOCHONDRIA AND TISSUE SLICES OF RAT RENAL CORTEX. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-85/157.
Description:
Slices of rat renal cortex were shown to take up Pb2+ during incubation in vitro; Pb2+ was also shown to enter mitochondria within the slices. The uptake of Pb2+ by isolated mitochondria was inhibited by N3-, La3+ and ruthenium red. A steady state of uptake was attained within 60 sec. The concentration dependence of uptake was complex; maximum uptake was attained at 25 micromols and inhibition ensued at higher concentrations. A substantial inhibitor-resistant component of Pb2+ uptake was noted, especially at medium Pb2+ concentrations greater than 25 micromoles, and these concentrations also inhibited respiration state 3. The effects on respiration were reduced if the mitochondria had been preincubated with ruthenium red.