You are here:
OXYGEN CONSUMPTION MEASURED WITH MICROCOMPUTER-ASSISTED WARBURG MANOMETRY
Citation:
Johnson, T., R. Mercer, P. Taylor, J. Graham, AND J. O'Neil. OXYGEN CONSUMPTION MEASURED WITH MICROCOMPUTER-ASSISTED WARBURG MANOMETRY. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-82/022 (NTIS PB84173152).
Description:
The authors have developed and tested an automated system that measures in vitro oxygen consumption by Warburg manometry in as many as 16 units that are under the simultaneous control of a microcomputer which requires attention at the beginning of the study only. The all-glass Submarine Volumometers used are readily adapted to automation using a microcomputer that interacts with an infrared photodetector sensitive to manometric changes in the reaction vessel and a stepper motor that can advance the calibrated micrometer in response to these changes. The microcomputer interacts with the user at the start of the study during data entry and subsequently determines volume changes related to oxygen consumption, calculates respiration rates, and prints or graphs the results without further user interaction.