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NEW METHOD FOR EXCYSTATION OF GIARDIA
Citation:
Sauch, J. NEW METHOD FOR EXCYSTATION OF GIARDIA. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-87/046 (NTIS PB87145918).
Description:
In vitro excystation of Giardia is used to estimate cyst viability and may also be used to obtain trophozoites and cyst walls for analysis. Recently published excystation procedures include the use of trypsin, serum or bile salts in the excystation step. Trypsin, serum or bile salts in the excystation medium was replaced by proteose peptone for G. muris and phytone peptone for G. lamblia. Comparison between this method utilizing peptones and that of Schaefer utilizing trypsin showed no significant differences in percent excystation for both G. muris and G. lamblia.
Record Details:
Record Type:DOCUMENT( REPORT )
Product Published Date:05/24/2002
Record Last Revised:04/16/2004
Record ID:
29768