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THE RESPONSE OF A HUMAN BRONCHIAL EPITHELIAL CELL LINE TO HISTAMINE: INTRACELLULAR CALCIUM CHANGES AND EXTRACELLULAR RELEASE OF INFLAMMATORY
Citation:
Noah, T., A. Paradiso, M. Madden, K. McKinnon, AND R. Devlin. THE RESPONSE OF A HUMAN BRONCHIAL EPITHELIAL CELL LINE TO HISTAMINE: INTRACELLULAR CALCIUM CHANGES AND EXTRACELLULAR RELEASE OF INFLAMMATORY. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/270.
Description:
The contribution of airway epithelium-derived factors to inflammation and tissue repair is unclear. ecause human airway epithelia are infrequently available for in vitro studies, transformed epithelial cell lines are of interest as models. e therefore investigated the response of an SV-40/adenovirus-transformed human bronchial epithelial cell line (BEAS-2B) to histamine, a mediator with relevance for airway diseases. he intracellular calcium (Ca2+i) response to histamine (10-4M) was measured, using Fura-2 fluorescence and microspectrofluorimetry. istamine induced a transient increase in CA2+i which originated from intracellular sources; this effect was blocked by the H1-receptor blocker, diphenhydramine, suggesting that SEAS cells retain functioning histamine receptors. EAS cells were grown to confluence on microporous, collagen-coated filters so that vectorial release of soluble mediators could be measured. onolayers exposed to histamine for 30 minutes released interleukin-6 and fibronectin in the apical direction, in a dose-dependent manner. ittle eicosanoic production was induced by histamine, either in the apical or the basolateral direction, although SEAS cells appeared to constitutively produce small amounts of PGE21 LTC4, LTD4, and 15-HETE as measured by HPLC. However, these cells formed large amounts of eicosanoics in response to ozone exposure as a positive control. omparison of our data with published reports for human airway epithelia in primary culture suggest that the BEAS cell line is, in a number of respects, a relevant model for the study of airway epithelial responses to a variety of stimuli.