EPA Science Inventory

Characterization of clinical and environmental Mycobacterium avium spp. isolates and their interaction with human macrophages

Citation:

Guirado, E., J. Arcos, R. Knaup, R. Reeder, B. Betz, C. Cotton, T. Patel, S. Pfaller, J. Torrelles, AND L. Schlesinger. Characterization of clinical and environmental Mycobacterium avium spp. isolates and their interaction with human macrophages. PLoS ONE . Public Library of Science, San Francisco, CA, 7(9):e45411, (2012).

Description:

Members of the Mycobacterium avium complex (MAC) are naturally occurring bacteria in the environment. A link has been suggested between M. avium strains in drinking water and clinical isolates from infected individuals. There is a need to develop new screening methodologies that can identify specific virulence properties of M. avium isolates found in water that predict a level of risk to exposed individuals. In this work we have characterized 15 clinical and environmental M. avium spp. isolates provided by the US Environmental Protection Agency (EPA) to improve our understanding of the key processes involved in the binding, uptake and survival of these isolates n primary human macrophages. M. avium serovar 8 was predominant among the isolates studied. Different amounts and exposure of mannose-capped lipoarabinomannan (ManLAM) and glycopeptidolipids (GPLs), both major mycobacterial virulence factors, were found among the isolates studied. Reference clinical isolate 104 serovar 1 and clinical isolates 11 and 14 serovar 8 showed an increased growth in primary human macrophages. The other isolates varied in their survival in these cells. Thus we conclude that the amounts of cell envelope ManLAM and GPL, as well as GPL serovar specificity are not the only important bacterial factors for dictating the early interactions of M. avium with human macrophages.

Purpose/Objective:

The goal of this study was to develop a tool to measure the attachment, uptake and replication of M. avium clinical and environmental isolates in human macrophages.

URLs/Downloads:

journal.pone.0045411   Exit

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Start Date: 05/22/2014
Completion Date: 05/22/2014
Record Last Revised: 06/03/2014
Record Created: 05/22/2014
Record Released: 05/22/2014
OMB Category: Other
Record ID: 276521

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL EXPOSURE RESEARCH LAB

MICROBIOLOGICAL AND CHEMICAL EXPOSURE ASSESSMENT DIVISION

MICROBIAL EXPOSURE RESEARCH BRANCH