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Zebrafish Development: High-throughput Test Systems to Assess Developmental Toxicity
Citation:
Padilla, S. Zebrafish Development: High-throughput Test Systems to Assess Developmental Toxicity. Edition 1, Chapter 20, Pablo Steinberg (ed.), High-throughput Screening Methods in Toxicity Testing. John Wiley & Sons Incorporated, New York, NY, , 371-383, (2013).
Impact/Purpose:
What is the study? This is a book chapter that reviews large scale zebrafish developmental screening studies, and also discusses some of the advantages and challenges of such studies. Why was it done? It was done because I was asked to contribute a chapter on zebrafish developmental screening to a book on high-throughput screening. What is the impact on the field and to the Agency? There is very little original impact as this work does not represent any original research. There are, however, some interesting !iummaries, especially in the last section where by summarizing the literature, we were able to derive a formula that estimated the internal dose of a chemical based solely on the chemical's LogP. This may be used in the future to estimate internal dose to the developing fish in order to equate dose among species or dosing regimens.
Description:
Abstract Because of its developmental concordance, ease of handling and rapid development, the small teleost, zebrafish (Danio rerio), is frequently promoted as a vertebrate model for medium-throughput developmental screens. This present chapter discusses zebrafish as an alternative animal model for screening for developmental toxicity and the different approaches that are taken by various investigators. Even though the zebrafish model is showing promise, there are still some aspects of the approach that may limit its usefulness for predictive toxicity.