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An Evaluation of Gestational Exposure to Perfluorooctanoic Acid (PFOA): Effects on Body Composition and Physiological Factors
Diggs, D., J. Norwood, C. Wood, M. Lin, J. Rogers, C. Lau, AND B. Abbott. An Evaluation of Gestational Exposure to Perfluorooctanoic Acid (PFOA): Effects on Body Composition and Physiological Factors. Presented at Society of Toxicology, Phoenix, AZ, March 23 - 27, 2014.
Exposure to environmental pollutants can be a factor for induction of metabolic disorders. This study examined if exposure to PFOA during development could alter body composition and other physiological outcomes. Study 1: Pregnant CD-1 mice were gavaged with PFOA at 0,0.001,0.01, 0.1, or 0.3 mg/kg body weight (bw) from gestation day (GD) 1 — 17. At weaning, pups were fed a high fat (HFD) or control (CD) diet. Body composition, blood pressure (bp), and gene expression in tissues of offspring were examined. Male- BW increased, in 0 mg PFOA+HFD vs 0 m PFOA+CD and 0.01 mg PFOA+HFD vs 0.01 mg PFOA+CD. In HFD, bw decreased in 0.3 vs 0 mg PFOA. There were no effects on percent of body fat. At postnatal day (PND) 90, diastolic bp was decreased in 0.1 and 0.3 mg PFOA+HFD vs 0 mg PFOA+HFD and increased in 0.3 mg PFOA+HFD vs 0.3 mg PFOA+CD. The bp effects of 0.1 mg PFOA+HFD persisted to PND 180. Female- At 0 and 0.001 mg PFOA+HFD had increased weight gain vs CD. The %fat increased in 0.001 vs 0 mg PFOA+HFD. At PND 180, diastolic bp decreased in 0.01 and 0.3 mg PFOA+CD vs 0 mg PFOA+CD. Differential gene regulation was produced by HFD and PFOA in white fat and liver at 52 weeks of age. At 0.001 mg PFOA+HFD vs 0.001 mg PFOA+CD, 3 genes in white fat and liver were under-expressed while 14 genes in white fat and 19 in liver were over expressed. At 0.01 mg PFOA+HFD vs 0.01 mg PFOA+CD, 3 genes in white fat and 4 genes in liver were under-expressed while 14 genes in white fat and 15 in liver were over-expressed. Study 2: Pregnant mice were fed NIH-31 (low phytoestrogen) diet and dosed with PFOA at 0, 0.01 0.1, or 1 mg/kg from GD 1 — 17. Pups remained on the NIH-31 diet. There were no age- or dose-related differences in bw or %body fat in either sex. In summary exposure to PFOA and HFD produced sex-dependent changes in bw, body composition, and bp response in mouse offspring exposed to the chemical during perinatal development. This abstract does not necessarily reflect USEPA policy.
This abstract will be presented at the Society of Toxicology meeting March 23-27, 2014, Phoenix, AZ.
Record Details:Record Type: DOCUMENT (PRESENTATION/ABSTRACT)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB
TOXICOLOGY ASSESSMENT DIVISION