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Optimization of Adenovirus 40 and 41 Recovery from Tap Water
Citation:
McMinn, B. Optimization of Adenovirus 40 and 41 Recovery from Tap Water. JOURNAL OF VIROLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 193(2):284-290, (2013).
Impact/Purpose:
Submit findings of adenovirus optimization experimentation to journal for publication
Description:
Currently, the U.S. Environmental Protection Agency’s Information Collection Rule (ICR) for the primary concentration of viruses from drinking and surface waters uses the 1MDS filter; however, a more cost effective option, the NanoCeram® filter, has been shown to recover comparable levels of enterovirus and norovirus from both matrices. In order to achieve the highest viral recoveries, filtration methods require the identification of optimal concentration conditions that are unique to each virus type. This study evaluated the effectiveness of 1MDS and NanoCeram filters in recovering adenovirus (AdV) 40 and 41 from tap water, and it optimized two secondary concentration procedures, the celite and organic flocculation method. During experimentation, adjustments in pH were made to both virus elution solutions and sample matrices to determine which resulted in higher virus recovery. Samples were analyzed by quantitative PCR (qPCR) and Most Probable Number (MPN) techniques and AdV recoveries were determined by comparing levels of virus in sample concentrates to that in the initial input. The recovery of adenovirus was highest for samples in unconditioned tap water (pH 8), with virus being concentrated via the 1MDS filter and the celite technique. Elution buffer containing 0.1% sodium polyphosphate at pH 10.0 was determined to be most effective overall for both AdV types. Under these conditions, the average recovery for AdV40 and 41 was 49% and 60%, respectively. By optimizing secondary elution steps, AdV recovery from tap water could be improved at least two-fold compared to the currently used methodology. Identification of the optimal concentration conditions for human AdV (HAdV) is important for timely and sensitive detection of these viruses from both surface and drinking waters.
