You are here:
Metabolomics from the Lab to the Field: Lessons Learned Along the Way
Citation:
Ekman, D., G. Ankley, J. Cavallin, J. Davis, E. Durhan, K. Jensen, M. Kahl, L. Makynen, D. Skelton, Q. Teng, Dan Villeneuve, AND Tim Collette. Metabolomics from the Lab to the Field: Lessons Learned Along the Way. Presented at 34th Annual SETAC North America, Nashville, TN, November 17 - 21, 2013.
Impact/Purpose:
see description
Description:
Use of metabolomics in laboratory studies for chemical toxicity evaluation is fast becoming an established technique in environmental science, displaying excellent sensitivity, physiological relevance, and providing valuable information regarding toxic mode(s)-of-action. These qualities, in addition to the lack of need for a sequenced genome, make a compelling case for the development and application of metabolomics to important ecological problems. However, a number of considerations must be weighed, both technical and biological in nature, throughout all aspects of a metabolomics study. Moreover, transitioning from lab-based studies to field application involves a myriad of additional complexities that can confound analyses. Our group is currently working to make this transition. This presentation will include a series of considerations and “lessons learned” for both laboratory-based studies and field application of metabolomics to environmentally relevant contaminants. Issues regarding study design, sample collection/handling, data acquisition and analyses will be discussed. In addition, considerations specific to conducting field-based metabolomics studies will be presented, along with examples from recent applications in which metabolomics was used to track impacts of complex environmental mixtures in fish.
