You are here:
Developmental Toxicity of Louisiana Crude Oiled Sediment to Zebrafish
Citation:
Barron, M., CrystalR Jackson, J. Krzykwa, Becky Hemmer, AND Sandy Raimondo. Developmental Toxicity of Louisiana Crude Oiled Sediment to Zebrafish. Presented at International Symposium on Polycyclic Aromatic Compounds, Corvallis, OR, September 08 - 12, 2013.
Impact/Purpose:
Determine dose response relationships between oil in sediment and fish development
Description:
Embryonic exposures to polycyclic aromatic hydrocarbons (PAHs) and petroleum products cause a characteristic suite of developmental defects in a variety of fish species. We exposed zebrafish embryos to sediment mixed with laboratory weathered South Louisiana crude oil. Oiled sediment exposures caused a reproducible set of developmental malformations in zebrafish embryos including yolk sac and pericardial edema, hemorrhaging, craniofacial and spinal defects, and tissue degeneration. Dose-response studies showed that total PAH concentrations of 23 mg/Kg (dry weight, 1% organic carbon normalized; 0.6% oil in sediment) caused a significant increase in defects, and 58 mg/Kg caused nearly complete embryo mortality (1.5% oil in sediment). This study demonstrated that oiled sediment is an exposure pathway to fish that can affect embryogenesis consistent with PAH mediated developmental toxicity.