You are here:
Evaluation of methods using celite to concentrate norovirus, adenovirus and enterovirus from wastewater
Citation:
Brinkman, N., T. Haffler, J. Cashdollar, AND E. Rhodes. Evaluation of methods using celite to concentrate norovirus, adenovirus and enterovirus from wastewater. JOURNAL OF VIROLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 193(1):140-146, (2013).
Impact/Purpose:
To disseminate results of research in the peer-reviewed scientific community.
Description:
Enteroviruses, noroviruses and adenoviruses are among the most common viruses infecting humans worldwide. These viruses are shed in the feces of infected individuals and can accumulate in wastewater. Therefore, wastewater is a source of a potentially diverse group of enteric viruses that can be used in many research applications. In this study, two procedures were evaluated to concentrate noroviruses, adenoviruses and enteroviruses from primary effluent of wastewater. In the first procedure, indigenous enteroviruses, noroviruses and adenoviruses were concentrated using celite (diatomaceous earth) followed by centrifugation through a 30K MWCO filter and nucleic acid extraction. The second procedure used celite concentration followed by nucleic acid extraction only. Enteric viruses were measured using RT-qPCR or qPCR and resulting recovery efficiencies were compared. A second set of primary effluent samples were seeded with 1000 PFU of coxsackievirus A7, coxsackievirus B1, poliovirus 1 or enterovirus 70 before concentration and processed through both procedures as described for evaluation of recovery of species representatives of enteroviruses. The pairing of the single step extraction procedure with the celite concentration process resulted in 47-98% recovery of examined viruses. However, the celite concentration process plus additional centrifugal concentration before nucleic acid extraction showed only 14-47% recovery of the targeted viruses. The celite concentration process followed by a large volume nucleic acid extraction technique proved to be effective at recovering these important human pathogens from wastewater samples.
