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Multi-Laboratory Validation of Estrone (E1) ELISA Methods
Citation:
Rubio, F., D. Russell, E. Kleiner, A. Tang, A. Lincoff, L. Webb, B. Kim, AND R. Schneider. Multi-Laboratory Validation of Estrone (E1) ELISA Methods. Presented at PittConn, Philadelphia, PA, March 17 - 21, 2013.
Impact/Purpose:
To inform the scientific community.
Description:
This project is a round-robin evaluation of commercially available Enzyme-Linked Immunosorbent Assay (ELISA) technology to quantitatively or qualitatively measure the hormone estrone (E1) in combined animal feeding operation (CAFO) receiving streams. ELISA is meant to be a simple, user-friendly technology that uses competition between enzyme labeled conjugates and the target analyte in the sample to bind with a limited number of antibody binding sites on a plate. The target compound concentration is determined by a color change measured on a standard plate reader.
