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Acute triadimefon-induced changes in the EEG of Long-Evans Rats
Citation:
Lyke, D., Kathy Mcdaniel, V. C. Moser, AND D. Herr. Acute triadimefon-induced changes in the EEG of Long-Evans Rats. Presented at Society of Toxicology, March 10 - 14, 2013.
Impact/Purpose:
This abstract will be presented at the Society of Toxicology Meeting, March 10-14, 2013, San Santonio, Tx
Description:
We have reported that the non-stimulus driven EEG is altered differently by acute treatment with deltamethrin, permethrin, fipronil, or imidacloprid (Lyke and Herr, Lyke et al., Toxicologist, 2010, 2011, 2012) in non-restrained animals. In the current study, we examined the ability to detect changes in EEG activity produced by triadimefon, a triazole pesticide which inhibits sterol synthesis in fungal cell membranes and inhibits dopamine re-uptake in the mammalian nervous system. Adult male Long-Evans rats were implanted with epidural screw electrodes. After about 1 week recovery, non-restrained animals were gavaged with corn oil (vehicle) and tested for 2 days for acclimation. On day 3, the rats were dosed with 1 ml/kg corn oil, 75, or 150 mg/kg triadimefon and tested 1 h later. EEG was recorded as 30 segments of 2 s durations, transformed using a Fast Fourier Transformation, and the spectra averaged. These dosages have increased motor activity in Long-Evans rats in previous studies. Treatment with 150 mg/kg triadimefon increased the amplitude associated with Theta activity compared to controls (31% increase), and compared to 75 mg/kg triadimefon (27% increase), when recorded between the visual cortex and cerebellum. These results were different from the decreased gamma activity resulting from treatment with fipronil, increased gamma activity following treatment with permethrin, slightly decreased alpha activity with deltamethrin, and lack of changes after dosing with imidacloprid. The data show that triadimefon can alter CNS activity as measured by EEG, and the alterations may differ from some other pesticides. This is an abstract of a proposed presentation and does not necessarily reflect EPA policy.