EPA Science Inventory

Metagenomic Analyses of Drinking Water Receiving Different Disinfection Treatments

Citation:

GOMEZ-ALVAREZ, V., R. P. REVETTA, AND J. W. SANTO-DOMINGO. Metagenomic Analyses of Drinking Water Receiving Different Disinfection Treatments. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 78(17):6095-6102, (2012).

Description:

A metagenome-based approach was utilized for assessing the taxonomic affiliation and function potential of microbial populations in free chlorine (CHL) and monochloramine (CHM) treated drinking water (DW). A total of 1,024, 242 (averaging 544 bp) and 849, 349 (averaging 554 bp) pyrosequencing reads were generated for the CHL and CHM, respectively. Most annotated proteins were found to be of bacterial origin (i.e., 90%), although eukaryotic, archaeal, and viral proteins were also identified. Differences in community structure and function were noted. Most notably, Legionella-like genes were more abundant in the CHL sample, while mycobacterial genes were more abundant in CHM. Genes associated with multiple disinfectant mechanisms were identified in both communities. Moreover, sequences linked to virulence factors, such as antibiotic resistance mechanisms, were observed in both microbial communities. This study provides new insights on the genetic network and potential biological processes associated with the molecular microbial ecology of DW microbial communities.

Purpose/Objective:

This study provides new insights on the genetic network and potential biological processes associated with the molecular microbial ecology of DW microbial communities.

URLs/Downloads:

Metagenomic Analy of DW Rec Diff Disinfec Trtmt   Exit

Website   Exit

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Start Date: 09/01/2012
Completion Date: 09/01/2012
Record Last Revised: 10/31/2012
Record Created: 10/26/2012
Record Released: 10/26/2012
OMB Category: Other
Record ID: 247171

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL RISK MANAGEMENT RESEARCH LABORATORY

WATER SUPPLY AND WATER RESOURCES DIVISION

MICROBIAL CONTAMINANTS CONTROL BRANCH