EPA Science Inventory

Utilizing Pyrosequencing and Quantitative pCR to Characterize Fungal Populations among House Dust Samples

Citation:

Nonnenmann, M. W., G. Coronado, B. Thompson, W. C. Griffith, J. D. Hanson, S. J. VESPER, AND E. M. Faustman. Utilizing Pyrosequencing and Quantitative pCR to Characterize Fungal Populations among House Dust Samples. JOURNAL OF ENVIRONMENTAL MONITORING. Royal Society of Chemistry, Cambridge, Uk, 14(8):2038-2043, (2012).

Description:

Molecular techniques are an alternative to culturing and counting methods in quantifying indoor fungal contamination. Pyrosequencing offers the possibility of identifying unexpected indoor fungi. In this study, 50 house dust samples were collected from homes in the Yakima Valley, WA. Each sample was analyzed by quantitative PCR (QPCR) for 36 common fungi and by fungal tag-encoded flexible (FLX) amplicon pyrosequencing (fTEFAP) for these and additional fungi. Only 24 of the samples yielded amplified results using fTEFAP but QPCR successfully amplified all 50 samples. Over 450 fungal species were detected by fTEFAP but most were rare. Twenty-two fungi were found by fTEFAP to occur with at least an average of ≥0.5% relative occurrence. Many of these fungi seem to be associated with plants, soil or human skin. Combining fTEFAP and QPCR can enhance studies of fungal contamination in homes.

Purpose/Objective:

To be entered

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Start Date: 08/01/2012
Completion Date: 08/01/2012
Record Last Revised: 05/09/2013
Record Created: 09/13/2012
Record Released: 09/13/2012
OMB Category: Other
Record ID: 246314

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL EXPOSURE RESEARCH LAB

MICROBIOLOGICAL AND CHEMICAL EXPOSURE ASSESSMENT DIVISION

MICROBIAL EXPOSURE RESEARCH BRANCH