EPA Science Inventory

Genomic sequences of two novel Levivirus single-stranded RNA coliphages (family Leviviridae): Evidence for recombination in environmental strains

Citation:

Friedman, S., W. Snellgrove, AND F. Genthner. Genomic sequences of two novel Levivirus single-stranded RNA coliphages (family Leviviridae): Evidence for recombination in environmental strains. Viruses. MDPI AG, Basel, Switzerland, 4(9):1548-1568, (2012).

Description:

Bacteriophages are likely the most abundant entities in the aquatic environment, yet knowledge of their ecology is limited. During a fecal source-tracking study, two genetically novel Leviviridae strains were discovered. Although the novel strains were isolated from coastal waters 1130 km apart (North Carolina and Rhode Island), these strains shared 97% nucleotide similarity and 97-100% amino acid similarity. When the novel strains were compared to nine Levivirus genogroup I strains, they shared 95-100% similarity among the maturation, capsid and lysis proteins, but only 84-85% in the RNA-dependent RNA polymerase gene. Further bioinformatic analyses suggested a recombination event occurred. To the best of our knowledge, this is the first description of viral recombinants in environmental Leviviridae ssRNA bacteriophages.

Purpose/Objective:

New scientific information regarding environmental bacteriophages and their genetic organization. Bacteriophages are the most abundant entity in the aquatic environment and knowledge of their ecology improves our understanding of ecological processes.

URLs/Downloads:

http://www.mdpi.com/1999-4915/4/9/1548   Exit

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Start Date: 09/13/2012
Completion Date: 09/13/2012
Record Last Revised: 10/22/2012
Record Created: 09/13/2012
Record Released: 09/13/2012
OMB Category: Other
Record ID: 246312

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB

GULF ECOLOGY DIVISION

ECOSYSTEM DYNAMICS AND EFFECTS BRANCH