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Performance of two quantitative PCR methods for microbial source tracking of human sewage and implications for microbial risk assessment in recreational waters
Citation:
Staley, C., K. V. Gordon, M. E. SCHOEN, AND V. J. Harwood. Performance of two quantitative PCR methods for microbial source tracking of human sewage and implications for microbial risk assessment in recreational waters. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 78(20):7317-7326, (2012).
Impact/Purpose:
to inform the public.
Description:
Before new, rapid quantitative PCR (qPCR) methods for recreational water quality assessment and microbial source tracking (MST) can be useful in a regulatory context, an understanding of the ability of the method to detect a DNA target (marker) when the contaminant soure has been diluted in environmental waters is needed. This study determined the limtis of detection and quantification of the human-associated Bacteroides sp. (HF183) and human polyomaviruses (HPyVs) qPCR methods for sewage diluted in buffer and in five ambient, Florida water types (estuarine, marine, tannic, lake and river).
