EPA Science Inventory

Use of Acid Treatment and a Selective Medium to Enhance the Recovery of Francisella tularensis from Water

Citation:

Humrighouse, B. W., N. J. ADCOCK, AND E. W. Rice. Use of Acid Treatment and a Selective Medium to Enhance the Recovery of Francisella tularensis from Water. Sept 2011 APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 77(18):6729-6732, (2011).

Description:

Journal article

Purpose/Objective:

Francisella tularensis has been associated with naturally occurring waterborne outbreaks and is also of interest as a potential biological wespon. Recovery of this pathogen from water using cultural methods is challenging due to the organism's growth requirements and interference by indigenous bacteria. An acid treatment procedure prior to culture on a selective agar was evaluated for recovery of F. tularensis from seeded water samples. The acid treatment procedure coupled with plating on cystine heart agar with rabbit blood and antiibiotics (CHARBab) allowed for the isolation of F. tularensis seeded to five natural water samples.

URLs/Downloads:

USE OF ACID TREATMENT AND A SELECTIVE MEDIUM TO ENHANCE THE RECOVERY OF FRANCSELLA TULARENSIS FROM WATER   Exit

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Start Date: 07/29/2011
Completion Date: 07/29/2011
Record Last Revised: 05/17/2013
Record Created: 07/14/2011
Record Released: 07/14/2011
OMB Category: Other
Record ID: 236814

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HOMELAND SECURITY RESEARCH CENTER

WATER INFRASTRUCTURE PROTECTION DIVISION