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Assessing Locomotor Activity in Larval Zebrafish: Influence of Extrinsic and Intrinsic Variables
PADILLA, S. J., D. L. HUNTER, B. K. PADNOS, S. Fraday, AND R. C. MACPHAIL. Assessing Locomotor Activity in Larval Zebrafish: Influence of Extrinsic and Intrinsic Variables. NEUROTOXICOLOGY AND TERATOLOGY. Elsevier Science Ltd, New York, NY, 33(6):624-630, (2011).
The U.S. Environmental Protection Agency is evaluating methods to screen and prioritize large numbers of chemicals for developmental toxicity. We are exploring methods to detect developmentally neurotoxic chemicals using zebrafish behavior at 6 days of age. The behavioral paradigm simultaneously tests individual larval zebrafish under both light and dark conditions in a 96-well plate using a video tracking system. We have found that many variables affect the level or pattern of locomotor activity, including age of the larvae, size of the well, and the presence of malformations. Some other variables, however, do not appear to affect larval behavior including type of rearing solution (10% Hank's vs. 1:3 Danieau vs 60 mg/kg Instant Ocean vs 1x and 1:10 x EPA Moderately Hard Water). Zebrafish larval behavior using a microtiter plate format may be an ideal endpoint for screening developmentally neurotoxic chemicals, but it is imperative that many test variables be carefully specified and controlled.
This is a study of the effects of different intrinsic and extrinsic variables on measuring function (i.e., locomotor activity) in very young Zebrafish. It was done to determine if these variables affected locomotor activity in the zebrafish.
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB
INTEGRATED SYSTEMS TOXICOLOGY DIVISION
GENETIC AND CELLULAR TOXICOLOGY BRANCH