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Human fecal source identification with real-time quantitative PCR
Citation:
SHANKS, O. C., L. PEED, M. SIVAGANESAN, R. A. HAUGLAND, AND E. C. CHERN. Human fecal source identification with real-time quantitative PCR. 2nd, Chapter 7, Paulsen I., Holmes A. (ed.), Environmental Microbiology, Methods in Molecular Biology Series. Humana Press Incorporated, Totowa, NJ, 1096:85-99, (2014).
Impact/Purpose:
To inform the public of a real-time quantitative PCR (qPCR) method that targets a Bacteroides dori human-associated genetic marker for the identification of human fecal pollution in ambient water samples.
Description:
Waterborne diseases represent a significant public health risk worldwide, and can originate from contact with water contaminated with human fecal material. We describe a real-time quantitative PCR (qPCR) method that targets a Bacteroides dori human-associated genetic marker for the identification of human fecal pollution in ambient water samples. The following protocol includes water sample collection, filtration, DNA isolation with a sample processing control, qPCR amplification with an internal amplification control (IAC), and data analysis. https://doi.org/10.1007/978-1-62703-712-9_7