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Evaluation of Hollow-Fiber Ultrafiltration Primary Concentration of Pathogens and Secondary Concentration of Viruses from Water
Citation:
RHODES, E., D. W. Hamilton, M. J. SEE, AND L. J. WYMER. Evaluation of Hollow-Fiber Ultrafiltration Primary Concentration of Pathogens and Secondary Concentration of Viruses from Water . JOURNAL OF VIROLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 176(1-2):38-45, (2011).
Impact/Purpose:
The overarching objective of this task is to provide Agency scientists and others the methods they need to measure the occurrence of waterborne viral pathogens. The method improvements will facilitate the development of risk-based assessments and tools used by the Agency to set regulations, policies and priorities for protecting human health.
Description:
In this study, tangential hollow-fiber ultrafiltration (HFUF) was evaluated for virus and Cryptosporidium parvum concentration. Recovery of viruses at a low filtration rate was found to be significantly greater than at a higher filtration rate, with the recoveries of bacteriophage MS2 at high and low filtration rates shown to be 64.7% and 98.7%, respectively. Poliovirus recoveries from tap water were similar to MS2, with recoveries of 62.9% and 104.5% for high and low filtration rates, respectively. C. parvum, which was only tested at high filtration rates, had an average recovery was 105.1%. In addition to the optimization of the primary concentration technique, this study also compared several secondary concentration procedures. The highest recovery (89.5%) of poliovirus from tap water concentrates was obtained when a beef extract-celite method was used and the virus was eluted from the celite with phosphate buffered saline, pH 9.0. When HFUF primary concentration and the optimal secondary concentration methods were combined, an average recovery of 89.3% was achieved for poliovirus. This study demonstrated that HFUF primary concentration method is effective at recovering MS2, poliovirus and C. parvum from large volumes of water and that beef extract-celite method is an effective secondary concentration method for the poliovirus tested.