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Macrophages Under Low Oxygen Culture Conditions Respond to Ion Parametric Resonance Magnetic Fields
Citation:
BLACKMAN, C. F., S. J. Fox, AND H. O. Owegi. Macrophages Under Low Oxygen Culture Conditions Respond to Ion Parametric Resonance Magnetic Fields. Presented at Rocky Mountain Bioengineering Symposium (48th Int'l) ISA Biomedical Sciences Instrumentaiton Symposium, Denver, CO, April 14 - 17, 2010.
Impact/Purpose:
In this study, we use this low-oxygen, inflammatory macrophage model to test its response to low-frequency magnetic fields
Description:
Macrophages, when entering inflamed tissue, encounter low oxygen tension due to the impairment of blood supply and/or the massive infiltration of cells that consume oxygen. Previously, we showed that such macrophages release more bacteriotoxic hydrogen peroxide (H202) when exposed in vitro to low oxygen than when cultured at usual ambient oxygen conditions. In this study, we use this low-oxygen, inflammatory macrophage model to test its response to low-frequency magnetic fields. Low-frequency fields are clinically used for bone and wound healing and are emerging as therapy for inflammatory diseases such as arthritis. The acceptance of these non-invasive therapies is slow due to the lack of knowledge of the cellular targets for magnetic fields. One possibility of such targets is biologically relevant ions. The Ion Parametric Resonance (IPR) model predicts specific externally applied AC and DC magnetic fields to resonate with the cyclotron motion of ions. This model is supported by experimental evidence, especially on a neuronal cellline.Using ou rmacrophage model, we tested AC and DC magnetic fields attitude ratio and frequency predicted by the IPR model for resonance with hydrogen, magnesium and manganese ions. Under these conditions, we found a significant increase in H202 release compared to control cells. Magnetic field exposure conditions in which parameters differed from the predictions of the IPR model showed no or less difference with the control cultures. These data indicate that magnetic fields can enhance H202 release of inflammatory macrophages, which is consistent with the predictions of the IPR model. [Abstract does not necessarily reflect the policies of the U.S. EPA.]