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Impacts of an Anti-androgen and an Androgen/anti-androgen Mixture on the Metabolite Profile of Male Fathead Minnow Urine
Citation:
COLLETTE, T. W., Q. TENG, K. M. JENSEN, M. D. KAHL, E. A. MAKYNEN, E. J. DURHAN, DAN VILLENEUVE, D. MARTINOVIC-WEIGELT, G. T. ANKLEY, AND D. R. EKMAN. Impacts of an Anti-androgen and an Androgen/anti-androgen Mixture on the Metabolite Profile of Male Fathead Minnow Urine. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, 44(17):6881-6886, (2010).
Impact/Purpose:
These results suggest that male FHM urinary metabolite profiling may be useful for directly detecting effects of anti-androgens.
Description:
Male and female fathead minnows (FHM) were exposed via the water to cyproterone acetate (CA), a model androgen receptor (AR) antagonist. FHM were also exposed to 517b-trenbolone (TB), a model AR agonist, and to mixtures of TB with CA. The urine metabolite profile of male FHM exposed to a high concentration of CA was markedly different from that of controls, and this difference was less for male FHM co-exposed to the associated TB+CA mixture. The exposure to TB alone had almost no effect on the male urine profile. These results suggest that male FHM urinary metabolite profiling may be useful for directly detecting effects of anti-androgens. The occurrence of male-like nuptial tubercles in female FHM exposed to TB and the mixtures of TB and CA supported the metabolomics data.