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Screening Complex Effluents for Estrogenic Activity with the T47D-Kbluc Cell Bioassay: Assay Optimization and Comparison to In Vivo Responses in Fish
Citation:
WEHMAS, L. C., J. E. CAVALLIN, E. J. DURHAN, M. D. KAHL, D. MARTINOVIC, J. MAYASICH, T. TUOMINEN, DAN VILLENEUVE, AND G. T. ANKLEY. Screening Complex Effluents for Estrogenic Activity with the T47D-Kbluc Cell Bioassay: Assay Optimization and Comparison to In Vivo Responses in Fish . ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, 30(2):439-445, (2011).
Impact/Purpose:
These studies support the future use of the T47D-Kbluc assay to characterize the total estrogenic activity of complex mixtures.
Description:
The endocrine activity of complex mixtures of chemicals associated with wastewater treatment plant effluents, runoff from concentrated animal feeding operations (CAFOs), and/or other environmental samples can be difficult to characterize based on analytical chemistry. In vitro bioassays provide a useful complement to analytical measurements, as they can characterize the integrated mechanism-specific biological activity of complex mixtures without the need to identify and/or quantify all its constituents. This study focused on development of methods to characterize the total estrogenic activity of complex samples using the T47D-Kbluc assay. A novel sample preparation method that minimized sample alteration and confounding matrix effects was developed. The utility of the method was demonstrated through the characterization of several wastewater treatment plant effluent samples. Additionally, the in vitro characterization was compared with in vivo characterization of the estrogenic potency of the effluents in fish. These studies support the future use of the T47D-Kbluc assay to characterize the total estrogenic activity of complex mixtures.