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Isolation and characterization of 108 novel microsatellite loci for Atlantic coastal killifish (Fundulus heteroclitus)
Citation:
JACKSON, S. A., R. WANG, J. Whitehead, D. A. Roberts, D. Duvernell, D. E. NACCI, AND M. BAGLEY. Isolation and characterization of 108 novel microsatellite loci for Atlantic coastal killifish (Fundulus heteroclitus). Molecular Ecology Resources. Blackwell Publishing, Malden, MA, 10(6):1098-1105, (2010).
Impact/Purpose:
Biological resource conservation and management programs have benefitted greatly over the last decade from advances in population genetics research. The EPA, while having very similar goals to these programs, has yet to capitalize on the rapid advances in molecular population genetic methods. This research aims to evaluate the utility of population genetic measurements for describing the current condition and vulnerabilities of biological populations exposed to anthropogenic stressors.
Description:
We characterized 108 polymorphic microsatellite loci for the mummichog (Fundulus heteroclitus), an Atlantic coastal killifish. Allelic diversity among 26 individuals ranged between 2 and 15 alleles per locus, while expected heterozygosity ranged from 0.075 to 0.904. Significant linkage disequilibrium was found for five sets of loci, while 13 loci significantly departed from Hardy-Weinberg expectations. Congeners assessed for cross-species amplification segregated for between 7 and 16 polymorphic loci. These markers represent a significant resource for studies of the ecology and genetics of F. heteroclitus and related species.
URLs/Downloads:
Isolation and characterization of 108 novel microsatellite loci for Atlantic coastal killifish (Fundulus heteroclitus)