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Exposure Research

Impacts of an Anti-Androgen and an Adrogen/Anti-Androgen Mixture on the Metabolite Profile of Male Fathead Minnow Urine

Citation:

COLLETTE, T. W., Q. TENG, K. M. JENSEN, M. D. KAHL, E. A. MAKYNEN, E. J. DURHAN, D. VILLENEUVE, D. MARTINOVIC, N. D. Mueller, G. T. ANKLEY, AND D. R. EKMAN. Impacts of an Anti-Androgen and an Adrogen/Anti-Androgen Mixture on the Metabolite Profile of Male Fathead Minnow Urine. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, 44(17):6881-6886, (2010).

Description:

Male and female fathead minnows (Pimephales promelas, FHM) were exposed via water to 20 or 200 μg/L of cyproterone acetate (CA), a model androgen receptor (AR) antagonist. FHM were also exposed to 500 ng/L of 17β-trenbolone (TB), a model AR agonist, and to mixtures of TB with both concentrations of CA. The urine metabolite profile (as measured by 1H NMR spectroscopy) of male FHM exposed to the high concentration of CA was markedly different from that of controls, and this difference was less for males coexposed to the associated TB+CA mixture. The exposure to TB alone had almost no impact on the male urine profile. These results suggest that male FHM urinary metabolite profiling may be useful for directly detecting effects of anti-androgens. In contrast, the urinary profile of male FHM exposed to the lower concentration of CA was not very different from that of controls, but, unexpectedly, this difference was increased when coexposed to the associated TB+CA mixture. This suggests that TB with CA at the lower concentration impacts male FHM through an interactive effect possibly unrelated, or in addition, to AR antagonism. The relative occurrence of male-like nuptial tubercles in female FHM exposed to TB and to the mixtures of TB and CA supported the metabolomics data.

Purpose/Objective:

see description

URLs/Downloads:

Environmental Science & Technology   Exit

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Start Date: 09/01/2010
Completion Date: 09/01/2010
Record Last Revised: 11/02/2010
Record Created: 03/18/2010
Record Released: 03/18/2010
OMB Category: Other
Record ID: 220684

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL EXPOSURE RESEARCH LAB

ECOSYSTEMS RESEARCH DIVISION

PROCESSES & MODELING BRANCH

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