You are here:
Development of an Immunoassay and a Sol-Gel-Based Immunoaffinity Cleanup Method for Coplanar PCBs from Soil and Sediment Samples
Citation:
Altstein, M., O. Aziz, N. Skalka, A. Bronshtein, J. C. Chuang, AND J. M. VAN EMON. Development of an Immunoassay and a Sol-Gel-Based Immunoaffinity Cleanup Method for Coplanar PCBs from Soil and Sediment Samples. ANALYTICA CHIMICA ACTA. Elsevier Science Ltd, New York, NY, 675(2):138-147, (2010).
Impact/Purpose:
The National Exposure Research Laboratory′s (NERL) Human Exposure and Atmospheric Sciences Division (HEASD) conducts research in support of EPA′s mission to protect human health and the environment. HEASD′s research program supports Goal 1 (Clean Air) and Goal 4 (Healthy People) of EPA′s strategic plan. More specifically, our division conducts research to characterize the movement of pollutants from the source to contact with humans. Our multidisciplinary research program produces Methods, Measurements, and Models to identify relationships between and characterize processes that link source emissions, environmental concentrations, human exposures, and target-tissue dose. The impact of these tools is improved regulatory programs and policies for EPA.
Description:
Two polychlorinated biphenyls (PCB) enzyme linked immunosorbent assays (ELISAs) were developed using goat PCB purified immunoglobulin (IgG) antibodies (Abs). The IgGs exhibited the highest affinity toward PCB-77 (24 ng mL−1) with sensitivities in the range of 6–11 ng mL−1. The Abs cross-reacted with PCB-126 and the heptachlorodibenzofuran 1,2,3,4,6,7,8-HpCDF but not with PCB-169, PCB-118, Aroclor 1232, 1248, 1260 or the hexachlorodibenzofuran 2,3,4,6,7,8-HxCDF. The IgGs were also used to develop a sol–gel-based immunoaffinity purification (IAP) method for cleanup of PCB-126. Recovery efficiencies depended on the sol–gel formats; a 1:12 format resulted in the highest binding capacity. Net binding capacity ranged from 112 to 257 ng, and 90% of the analyte could be eluted with only 2mL of ethanol. The method was also very efficient in purifying PCB-126 from spiked soil and sediment samples from contaminated sites; and eliminating matrix interferences to a degree that enabled analysis of the purified samples by ELISA. The approaches developed in the course of the study form a basis for the development of additional IAP methods for other PCBs, and their implementation in high-throughput screening programs for PCB in food, soil, and other environmental and biological samples.
URLs/Downloads:
Development of an Immunoassay and a Sol-Gel-Based Immunoaffinity Cleanup Method for Coplanar PCBs from Soil and Sediment Samples (PDF, NA pp, 174 KB, about PDF)Analytica Chimica Acta
