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Toxicity comparison of chlorinated and brominated dibenzo-p-dioxins and dibenzofurans in industrial source samples by HRGC/HRMS and enzyme immunoassay
Citation:
SAMARA, F., B. Wyrzykowska, D. G. TABOR, D. TOUATI, AND B. K. GULLETT. Toxicity comparison of chlorinated and brominated dibenzo-p-dioxins and dibenzofurans in industrial source samples by HRGC/HRMS and enzyme immunoassay. ENVIRONMENT INTERNATIONAL. Elsevier Science Ltd, New York, NY, 36(3):247-253, (2010).
Impact/Purpose:
journal article, post publication
Description:
Limited information is available on the applicability of polychlorinated dibenzo-p-dioxin/furan (PCDD/F) toxicity assays to their brominated counterparts: polybrominated dibenzo-p-dixoins/furans (PBDDs/Fs). We estimated the toxicity of mixtures of chlorinated, brominated, and mixed bromochloro-dioxins and-furan laboratory standards using a chemically-activated luciferase gene expression cell bioassay. The relative effects potency values obtained were comparable to the World Health Organization (WHO) toxic equivalency factors (TEFs) and in agreement with the concept of additive cogener toxicity of mixtures of dioxins and furans. Enzyme immunoassay-based toxic equivalents, however, showed overestimation for the PCDDs/Fs (0-4 orders of magnitudes higher) and under estimation for PBDDs/Fs (0-1 orders of magnitude lower) when compared to high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS)-based TEQ calculation (using WHO TEFs) in samples from and industrial source line. No correlation was found between the EIA and the HRGC/HRMS data, which could be attributed to differences in homologue-specific cross-reactivity responses. HRMS data, which could be attributed to differences in homologue-specific cross-reactivity responses, sample matrix type, and presence of other compounds competing for antibody binding in the immunoassay.
URLs/Downloads:
NRMRL RTP P 1054.PDF (PDF, NA pp, 1047 KB, about PDF)TOXICITY COMPARISON OF CHLORINATED AND BROMINATED DIBENZO-P-DIOXINS AND DIBENZOFURANS IN INDUSTRIAL SOURCE SAMPLES BY HRGC/HRMS AND ENZYME IMMUNOASSAY
