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Influence of Ovarian Stage on Transcript Profiles in Fathead Minnow (Pimephales promelas) Ovary Tissue
Citation:
VILLENEUVE, DAN, N. GARCIA-REYERO, D. MARTINOVIC, J. E. CAVALLIN, N. D. MUELLER, L. C. WEHMAS, M. D. KAHL, A. L. LINNUM, E. J. PERKINS, AND G. T. ANKLEY. Influence of Ovarian Stage on Transcript Profiles in Fathead Minnow (Pimephales promelas) Ovary Tissue. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, 98:354-366, (2010).
Impact/Purpose:
The results provide key information which can aid the design and interpretation of future ecotoxicogenomics experiments. Additionally, they provide insights which can support the development and testing of novel hypotheses regarding regulation and control of asynchronous reproductive development, which may ultimately inform construction of biologically-based computational models of fish reproduction. Finally, the study identifies a number of putative molecular markers that may serve as indicators of female gonad stage/development in the absence of more costly and time-consuming histological analysis.
Description:
Small fish species with an asynchronous pattern of gonad development and reproduction are commonly used test organisms in aquatic ecotoxicology and more recently in ecotoxicogenomics. This study applies coordinated histological examination and fathead minnow oligonucleotide microarrays to study variations in the gene expression profiles in an asynchronous spawning fish species as a function of relative proportions of ovarian follicles in various stages of development. The results provide key information which can aid the design and interpretation of future ecotoxicogenomics experiments. Additionally, they provide insights which can support the development and testing of novel hypotheses regarding regulation and control of asynchronous reproductive development, which may ultimately inform construction of biologically-based computational models of fish reproduction. Finally, the study identifies a number of putative molecular markers that may serve as indicators of female gonad stage/development in the absence of more costly and time-consuming histological analysis. Overall the product complements on-going computational toxicology research with small fish models aimed at developing systems-oriented biological knowledge, molecular markers, and computational models that can inform ecological risk assessments.
