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Proteomic analyses of the environmental toxicity of carcinogenic chemicals
Citation:
GE, Y., M. E. BRUNO, K. WALLACE, W. M. WINNIK, AND S. NESNOW. Proteomic analyses of the environmental toxicity of carcinogenic chemicals. Presented at Society of Environmental Toxicology and Chemistry Annual Meeting (SETAC-EU), Seville, SPAIN, May 23 - 27, 2010.
Impact/Purpose:
To understand the toxicity pathways and/or modes ofaction for human health risk assessment, molecular characterization ofthe protein expression and posttranslational profiles is required for a complete understanding of chemical-induced toxic responses. The purpose ofthe study is to evaluate the ability ofproteomics data to predict toxicity, classify chemicals and identify relevant biological pathways and mechanisms oftoxicity.
Description:
Protein expression and posttranslational modifications consistently change in response to the exposure to environmental chemicals. Recent technological advances in proteomics provide new tools for more efficient characterization of protein expression and posttranslational modifications in cells and tissues exposed to the chemicals. To apply quantitative toxicoproteomic analysis to the evaluation of environmental toxicity and to the identification of toxic mechanisms and modes of action as well as to biomarkers of exposure, toxicity, and effect for human health risk assessment, we have developed an integrated toxicoproteomic platform. This platform has been applied to the analysis of the effects of several environmental carcinogens such as arsenic and conazoles on protein expression, phosphorylation, and oxidation in mouse liver and in human cells. This presentation will focus on the development of proteomics technologies and applications, to the identification of toxicity pathways, mechanisms of toxic action, and potential environmental biomarkers which are required and routinely used for human health risk assessment of environmental chemicals. In addition, the issues on how OMICS, especially toxicoproteomic data, can enhance our understanding of environmental diseases and increase the efficiency and accuracy of a risk assessment will also be discussed.