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A Novel Way To Identify Precursors That Degrade To Perfluourinated Compounds In Activated Sludge Using Ion-Trap Time-Of-Flight Mass Spectrometer
Citation:
Nakayama, S. F., J. B. Hines, AND M. A. MILLS. A Novel Way To Identify Precursors That Degrade To Perfluourinated Compounds In Activated Sludge Using Ion-Trap Time-Of-Flight Mass Spectrometer. Presented at SETAC New Orleans 30th Anniversary 2009, New Orleans, LA, November 19 - 23, 2009.
Impact/Purpose:
To present the details of Ion-Trap Time-Of-Flight Mass Spectrometer technique and identify the precursors that degrade to perfluorinated compounds in activated sludge.
Description:
An increasing number of studies have been conducted to investigate the environmental distribution of perfluorinated alkyl compounds (PFCs), many of which are known to be toxic in laboratory animals. Despite growing public concerns, the fate and transport of PFCs are little understood. Many studies have demonstrated wastewater treatment plants (WWTPs) as possible major contributors to the surface water environment. In order to investigate the impact of WWTPs on PFC load to the surface water environment, precursor compounds that potentially degrade to PFCs in WWTP process should be identified and quantified together with PFCs. Existing metabolite structural analysis software coupled with the Ion-Trap Time-Of-Flight (IT-TOF) mass spectrometry was modified and applied to extracts of WWTP samples. Samples were run on a high-performance liquid chromatograph equipped with IT-TOF, MSn spectra were obtained automatically by programming the instrument to acquire the MSn scan when each chromatographic peak hit a threshold. Each precursor was identified using accurate mass (< 5 ppm), MSn spectra, isotope pattern matching, carbon-hydrogen ratio, and nitrogen rule. Utilizing the software, spectra for precursor materials were extracted from large data sets and associated with final degraded PFCs. The details of the technique and identified precursors will be presented.
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Conference Information
A Novel Way To Identify Precursors That Degrade To Perfluorinated Compounds In Activated Sludge Using Ion-Trap Time-Of-Flight Mass Spectrometer (PDF, NA pp, 9 KB, about PDF)