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Pyrethroid insecticide accumulation in primary cultures of cortical neurons in vitro
Citation:
SHAFER, T. J., A. Jay, AND M. F. HUGHES. Pyrethroid insecticide accumulation in primary cultures of cortical neurons in vitro. Presented at Society of Toxicology 49th Annual Meeting, Salt Lake City, UT, March 07 - 11, 2010.
Impact/Purpose:
This data will be useful for making comparisons between in vivo and in vitro studies regarding effective concentrations of pyrethroids.
Description:
Primary cultures of neurons have been widely utilized to study the actions of pyrethroids and other neurotoxicants, with the presumption that the media concentration accurately reflects the dose received by the cells. However, recent studies have demonstrated that lipophilic compounds (e.g. methylmercury, PCBs and PBDEs) rapidly accumulate in cells to concentrations much higher than in the surrounding media. To test the hypothesis that pyrethroids rapidly accumulate in neurons in vitro, the time (0-90 min) and concentration (0.05 10 p.M) dependence of accumulation of eHl-deltamethrin (DM), eH]-bifenthrin (BF) and [14C]_ cis permethrin (PM) into primary cultures of cortical neurons was examined. Accumulation of all three pyrethroids was time-and concentration-dependent, with only small differences observed between the compounds. Concentration-dependent accumulation of PM and BF were similar, achieving a oftotal-0.25 nmol in cells after 30 min in a 10 p.M solution. DM accumulation was lower, reaching a maximum of 0.14 nmol after 30 min in a 10 p.M solution. In 1 p.M solutions, DM and PM content in cells was 0.039 and 0.038 nmol after 9Q min. At all concentrations and times, pyrethroid accumulation in cells was less than 3% ofthe total radioactivity applied. The amount of radioactivity recovered in the media at the end of incubation ranged from -75%98%; the remainder (0 to 25%) was presumed to bind to the plastic of the culture plates. These results demonstrate rapid time-and concentration-dependent accumulation ofpyrethroids in neurons in vitro. Further, for the three pyrethroids examined, there do not appear to be large differences in their accumulation. This data will be useful for making comparisons between in vivo and in vitro studies regarding effective concentrations of pyrethroids. (This abstract does not reflect EPA policy)