You are here:
Exposure of Three Generations of the Estuarine Sheepshead Minnow (Cyprinodon variegatus) to the Androgen 17β-trenbolone: Effects on Survival, Development, and Reproduction.
Citation:
CRIPE, G. M., BECKY L. HEMMER, S. RAIMONDO, L. R. GOODMAN, AND D. H. KULAW. Exposure of Three Generations of the Estuarine Sheepshead Minnow (Cyprinodon variegatus) to the Androgen 17β-trenbolone: Effects on Survival, Development, and Reproduction. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, 29(9):2079-2087, (2010).
Impact/Purpose:
The Agency is developing Tier II testing protocols for multiple generation exposures of a variety of taxa to endocrine disrupting chemicals to evaluate dose-response and latent or cumulative adverse effects. This study measures the traditional life-cycle endpoints in an exposure of sheepshead minnows through three generations to the androgenic metabolite 17B-trenbolone (T) and made comparisons among generations to identify early indicators of long-term effects or changes in sensitivity with exposure duration.
Description:
Estimating long-term effects of endocrine disrupting chemicals (EDCs) on a species is important to assessing the overall risk to the populations. This study reports the results of a 42-week exposure of estuarine sheepshead minnows (Cyprinodon variegatus) to the androgen, 17B-trenbolone (T) conducted to determine if partial (F0) or single-generation (F1) fish exposures identify multigenerational (F0 to F3) effects of androgens on fish. Adult F0 fish were exposed to 0.007, 0.027, 0.13, 0.87 and 4.1 ug T/L, the F1 generation to ≤ 0.87 ug T/L, the F2 fish to ≤ 0.13 ug T/L, and the F3 fish to < 0.027 ug T/L. The highest concentrations with reproducing populations at the end of the F0, F1 and F2 generations were 4.1, 0.87, and 0.027 ug T/L, respectively. Reproduction in the F0, F1, and F2 generations was significantly reduced at 0.87, 0.027, and 0.027 ug T/L, respectively. Fish were significantly masculinized in the F1 generation exposed to 0.13 ug T/L or greater. Female plasma vitellogenin was significantly reduced in F0 fish exposed to > 0.87 ug T/L. Gonadosomatic indices of the F0 and F1 generations were significantly increased at 0.87 and 0.13 ug T/L in the F0 and F1 generation, respectively, and were accompanied by ovarian histological changes. Reproduction was the most consistently sensitive measure of androgen effects and, after a life-cycle exposure, the daily reproductive rate predicted concentrations affecting successive generations. The present study provides evidence that a multiple generation exposure of fish to some EDCs can result in developmental and reproductive changes that have a much greater impact on the success of a species than was indicated from shorter term exposures.