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Low level ozone exposure induces airways inflammation and modifies cell surface phenotypes in healthy humans
Citation:
ALEXIS, N., J. C. LAY, M. HAZUCHA, B. HARRIS, M. L. Hernandez, P. A. BROMBERG, H. R. KEHRL, D. DIAZ SANCHEZ, C. S. KIM, R. B. DEVLIN, AND D. B. PEDEN. Low level ozone exposure induces airways inflammation and modifies cell surface phenotypes in healthy humans. INHALATION TOXICOLOGY. Taylor & Francis, Inc., Philadelphia, PA, 22(7):593-600, (2010).
Impact/Purpose:
Objective: The purpose of this study was to determine if exposure to 0.08 parts per million ozone for 6.6 hours induces inflammation and modifies immune cell surface phenotypes in the airways ofhealthy adult subjects
Description:
Background: The effects of low level ozone exposure (0.08 ppm) on pulmonary function in healthy young adults are well known, however much less is known about the inflammatory and immuno-modulatory effects oflow level ozone in the airways. Techniques such as induced sputum and flow cytometry make it possible to examine airways inflammatory responses and changes in immune cell surface phenotypes following low level ozone exposure. Objective: The purpose of this study was to determine if exposure to 0.08 parts per million ozone for 6.6 hours induces inflammation and modifies immune cell surface phenotypes in the airways ofhealthy adult subjects. Methods: Fifteen normal volunteers underwent an established 0.08 part per million ozone exposure protocol to characterize the effect ofozone on airways inflammation and immune cell surface phenotypes. Induced sputum and flow cytometry were used to assess these endpoints. Results: Exposure to 0.08 ppm ozone for 6.6 hours induced increased airway neutrophils,monocytes and dendritic cells and modified the expression ofCD14, HLA-DR, CD80 and CD86 on monocytes 24 hours following exposure Conclusion: Exposure to 0.08 parts per million ozone is associated with increased airways inflammation and promotion of antigen presenting cell phenotypes hours following exposure. Clinical Implications: These observations suggest that exposure to levels of ozone encountered in ambient air during the summer months may confer increased risk for exacerbation ofozone induced lung disease by promoting inflammation and antigen presenting phenotypes in the airway.
