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In Vitro Biocompatibility of Nanoscale Zerovalent Iron Particles (NZVI) Synthesized using tea-polyphenols.
Citation:
Nadagouda, M. N., A. B. Castle, R. C. Murdock, S. M. Hussain, AND R. S. Varma. In Vitro Biocompatibility of Nanoscale Zerovalent Iron Particles (NZVI) Synthesized using tea-polyphenols. GREEN CHEMISTRY. Royal Society of Chemistry, Cambridge, Uk, 12(1):114-122, (2010).
Impact/Purpose:
To inform the public
Description:
A “green” protocol was used for the rapid generation of nanoscale zerovalent iron (NZVI) particles using tea polyphenols. The NZVI particles were subsequently examined for in vitro biocompatibility using the human keratinocyte cell (HaCaT) line as a skin exposure model. The cells were exposed to (ZVI) nanoparticles for time periods of 24 and 48 hours. Assessing the biocompatibility involved using the methyl tetrazolium, or MTS, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)) and Lactate dehydrogenase (LDH) assays to determine in vitro cytotoxicity following a short exposure. The evaluation of mitochondrial function (MTS) and membrane integrity (LDH) in human keratinocytes showed that these “green” synthesized NZVI particles were nontoxic in the human keratinocytes exposed. In fact, in most cases, these “green” nanoparticles induced a prolific response in the cellular functions even at the highest concentration (100µg/ml).
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IN VITRO BIOCOMPATIBILITY OF NANOSCALE ZEROVALENT IRON PARTICLES (NZVI) SYNTHESIZED USING TEA POLYPHENOLS
In Vitro Biocompatibility of Nanoscale Zerovalent Iron Particles (NZVI) Synthesized using tea-polyphenols (PDF, NA pp, 719 KB, about PDF)