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Improved Cryptosporidium parvum oocysts propagation using dexamethasone suppressed CF-1 mice

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Citation:

WARE, M. W. AND E. VILLEGAS. Improved Cryptosporidium parvum oocysts propagation using dexamethasone suppressed CF-1 mice . Veterinary Parasitology. Elsevier, Shannon, Ireland, 168(3-4):329-331, (2010).

Impact/Purpose:

The overall objective of this task is the development of improved occurrence detection methods for protozoan parasites and Microsporidia. Since this work is a primary focus of the Branch, this task supports several individual projects related to sample preparation and protozoan detection. Together these projects will lead to complete methods able to support the UCMR and the CCL2 and CCL3.

Description:

This study evaluates Cryptosporidium parvum oocyst production in dexamethasone suppressed CF-1 and C57BL/6 mice. Both models can yield 1 x 109 total oocysts over a 20 day production period; however, only 20 CF-1 mice are required to reliably achieve this goal compared to 40 C57BL/6 mice. Although oocyst yields per mouse are similar for both mouse strains, the survival rate for CF-1 mice is higher, resulting in reduced lost production time per study when compared to the C57BL/6 mice. This study presents a more efficient and cost effective dexamethasone suppressed murine model of propagating high concentrations of C. parvum oocysts.

Record Details:

Record Type:DOCUMENT( JOURNAL/ PEER REVIEWED JOURNAL)
Product Published Date:03/25/2010
Record Last Revised:04/08/2010
OMB Category:Other
Record ID: 210167