Recovery Efficiency and Limit of Detection of Aerosolized B. anthracis Sterne from Environmental Surface Samples
Citation:
Estill, C. F., P. A. Baron, J. K. Beard, M. J. Hein, L. D. Larsen, L. Rose, F. W. SCHAEFER, J. Noble-Wang, L. Hodges, H. LINDQUIST, G. J. Deye, AND M. J. Arduino. Recovery Efficiency and Limit of Detection of Aerosolized B. anthracis Sterne from Environmental Surface Samples. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 75(13):4297-4306, (2009).
Impact/Purpose:
After the anthrax incidents in 2001 several surface sampling techniques for biological agents were found to be inadequately validated, especially at low surface loadings. We aerosolized Bacillus anthracis Sterne spores within a chamber to achieve very low surface loading (c. 0.03, 0.3 and 2 CFU/cm2). Coupons seeded in the chamber were sampled with swab, wipe or vacuum surface sampling methods and these coupons were analyzed at three laboratories.
Description:
journal article
URLs/Downloads:
RECOVERY EFFICIENCY AND LIMIT OF DETECTION OF AEROSOLIZED BACILLUS ANTHRACIS STERNE FROM ENVIRONMENTAL SURFACE SAMPLES