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Evaluation of the ToxCast Suite of Cellular and Molecular Assays for Prediction of In Vivo Toxicity
Citation:
HOUCK, K. A. Evaluation of the ToxCast Suite of Cellular and Molecular Assays for Prediction of In Vivo Toxicity. Presented at Society of Toxicology Annual Meeting, Baltimore, MD, March 15 - 19, 2009.
Impact/Purpose:
Patterns of activity for chemicals with similar in vivo activities will be compared and their effect on potential toxicity pathways evaluated. The utility of this approach to screening chemicals for human toxicity potential will be discussed.
Description:
Measurement of perturbation of critical signaling pathways and cellular processes using in vitro assays provides a means to predict the potential for chemicals to cause injury in the intact animal. To explore the utility of such an approach, a diverse collection of human in vitro cellular assays was used to profile the activity of a library of 309 bioactive compounds having known toxicities. Assays included both primary cells and cell lines as well as biotransformation capacity. In addition, effects of the chemicals on specific molecular targets was determined through screening in a broad panel of high-throughput biochemical assays to provide potential mechanism of action information.