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Predicting Virulence of Aeromonas Isolates Based-on Changes in Transcription of c-jun and c-fos in Human Tissue Culture Cells
Citation:
HAYES, S. L., M. Waltmann, M. J. DONOHUE, D. J. LYE, AND S. J. VESPER. Predicting Virulence of Aeromonas Isolates Based-on Changes in Transcription of c-jun and c-fos in Human Tissue Culture Cells. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, 107(3):964-969, (2009).
Impact/Purpose:
The objective of this project is to demonstrate the usefulness of the application of the standardized dust sampling protocol in conjunction with the DNA-based method of mold analysis and the resulting Environmental Relative Moldiness Index in projects across the US related to childhood asthma.
Description:
Aims: To assess virulence of Aeromonas isolates based on the change in regulation of c-jun and c-fos in the human intestinal tissue culture cell line Caco-2. Methods and Results: Aeromonas cells were added to Caco-2 cells at approximately a one to one ratio. After 1, 2 and 3 hours incubation at 37oC, mRNA was extracted from the cells and gene expression of two host genes, c-jun and c-fos, quantified. Aeromonas isolates which were virulent in the neonatal mouse model demonstrated up-regulation of c-jun and c-fos compared to avirulent isolates. Conclusions: Human cell culture results showed that c-jun and c-fos were predictive of Aeromonas virulence. Significance and Impact of the Study: An Aeromonas relative virulence scale (ARVS) is proposed for use in the testing of Aeromonas drinking water isolates.