APTAMER CAPTURE AND OPTICAL INTERFEROMETRIC DETECTION OF CYANOBACTERIAL TOXINS
Description:
Cyanobacterial toxins have been identified as a health risk in source and finished waters passing through drinking water utilities in the United States. In this project, a rapid, sensitive and field usable sensor based on an aptamer modified planar waveguide interferometric sensor will be developed to detect, identify and quantify types of cyanotoxins including the high priority neurotoxins anatoxin-a, cylindrospermopsin and microcystin-LR in source water.
Record Details:
Record Type:PROJECT(
ABSTRACT
)
Start Date:06/01/2008
Completion Date:04/30/2011
Record ID:
200625
Keywords:
CYANOBACTERIA, CYANOBACTERIAL TOXIN, APTAMER, INTERFEROMETRIC SENSOR,
Related Organizations:
Role
:OWNER
Organization Name
:GEORGIA INSTITUTE OF TECHNOLOGY
Mailing Address
:225 North Ave
Citation
:Atlanta
State
:GA
Zip Code
:30332
Project Information:
Approach
:Aptamers specific to anatoxin-a, cylindrospermopsin and microcystin-LR will be selected and evaluated. The aptamers will be used as recognition elements on waveguide’s sensing surface. The sensing assays will be characterized for their sensitivity, specificity, stability and matrix effects. Both unspiked and spiked water samples with the target toxins will be measured using the aptamer coated waveguide interferometric sensor. Commercial ELISA tests and/or a published protocol using LC/MS for the detection of cyanobacteria toxin will be used as a comparison to the waveguide results.
Cost
:$600,000.00
Research Component
:Drinking Water
Risk Paradigm
:RISK ASSESSMENT
Approach
:Aptamers specific to anatoxin-a, cylindrospermopsin and microcystin-LR will be selected and evaluated. The aptamers will be used as recognition elements on waveguide’s sensing surface. The sensing assays will be characterized for their sensitivity, specificity, stability and matrix effects. Both unspiked and spiked water samples with the target toxins will be measured using the aptamer coated waveguide interferometric sensor. Commercial ELISA tests and/or a published protocol using LC/MS for the detection of cyanobacteria toxin will be used as a comparison to the waveguide results.
Cost
:$600,000.00
Research Component
:OTHER
Risk Paradigm
:RISK ASSESSMENT
Approach
:Aptamers specific to anatoxin-a, cylindrospermopsin and microcystin-LR will be selected and evaluated. The aptamers will be used as recognition elements on waveguide’s sensing surface. The sensing assays will be characterized for their sensitivity, specificity, stability and matrix effects. Both unspiked and spiked water samples with the target toxins will be measured using the aptamer coated waveguide interferometric sensor. Commercial ELISA tests and/or a published protocol using LC/MS for the detection of cyanobacteria toxin will be used as a comparison to the waveguide results.
Cost
:$600,000.00
Research Component
:M/DBP (MICROBIAL)
Risk Paradigm
:RISK ASSESSMENT
Project IDs:
ID Code
:R833839
Project type
:EPA Grant