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A Mouse Model for Characterization of Gastrointestinal Colonization Rates Among Environmental Aeromonas Isolates
Citation:
LYE, D. J. A Mouse Model for Characterization of Gastrointestinal Colonization Rates Among Environmental Aeromonas Isolates. CURRENT MICROBIOLOGY. Springer, New York, NY, 58(5):454-458, (2009).
Impact/Purpose:
1. Identify animal based procedures that will be useful to better characterize infectivity in virulent strains of Helicobacter pylori. 2. Determine if short-term studies such as repeated intraperitoneal exposures or multiple gavage exposures can be used to determine the capacity of strains to colonize tissues (including the coccoid form). 3. Evaluate animal strains of Helicobacteras possible substitutes for H. pylori virulence capability in infectivity experiments and also as possible zoonotic sources of human infection.
Description:
The colonization rates of ten different environmental isolates of Aeromonas were determined using a novel mouse-streptomycin pre-treatment method. A novel streptomycin pre-treatment prepared animals with a transient alteration in colon flora that allowed colonization by Aeromonas which would not occur in the presence of normal competitive host bacteria. Colonization rates of Aeromonas salmonicida, Aeromonas enchelia, and Aeromonas allosaccharophila were low and occurred randomly with respect to concentrations of the dosage consumed by the animals. In contrast, Aeromonas hydrophila, Aeromonas veronii, and Aeromonas caviae exhibited relatively high rates of colonization of mouse colon tissues.