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Inactivation of Helicobacter pylori by Chloramination
Citation:
JOHNSON, CLIFFORD H. AND L. A. BOCZEK. Inactivation of Helicobacter pylori by Chloramination. Journal of Environmental Monitoring and Restoration. Society of Advocates for Sustainable Environment, Orangeburg, SC, 4:83-87, (2008).
Impact/Purpose:
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Description:
Three strains of Helicobacter pylori (H. pylori) were studied to determine their resistance to chloramination. H. pylori is an organism listed on the U.S. Environmental Protection Agency’s (USEPA) Contaminant Control List (CCL). H. pylori was exposed to 2ppm of pre-formed monochloramine at 5°C and 25°C. Viability of H. pylori was determined using the spread plate method onto two separate media, blood agar plates and Wilkins-Chalgren agar. Results from this study indicated that Wilkins-Chalgren agar was ineffective at recovering stressed H. pylori due to exposure of monochloramine. All three strains of H. pylori had a 3 log or better reduction after 20 minutes at 25°C, and after 30 minutes at 5°C on blood agar plates. The data in this study demonstrates that disinfection of H. pylori can be achieved by using monochloramine as a halogen disinfectant.
