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Determination of relative assay response factors for toxic chlorinated and bromiated dioxins/furans using an enyme immunoassay (EIA) and a chemically-activated luciferase gene expression cell bioassay (CALUX)
Citation:
SAMARA, F., B. K. GULLETT, R. O. Harrison, A. Chu, AND G. C. Clark. Determination of relative assay response factors for toxic chlorinated and bromiated dioxins/furans using an enyme immunoassay (EIA) and a chemically-activated luciferase gene expression cell bioassay (CALUX). ENVIRONMENTAL INTERNATIONAL. Pergamon Press Ltd., New York, NY, 35(3):588-593, (2009).
Impact/Purpose:
For journal Environmental International (Elsevier)
Description:
Determination of dioxin-like activity requires knowledge of both the concentration and toxicity to evaluate the risk of adverse human health and environmental effects. The dioxin-like response of several polybrominated dibenzo-p-dioxins/furans (PBDDs/Fs) and polybrominated/chlorinated dibenzo-p-dioxins/furans (PBCDDs/Fs) in comparison to polychlorinated dibenzo-p-dioxins/furans (PCDDs/Fs) was detected using a chemically-activated luciferase gene expression cell bioassay system (CALUX) and the antibody-based method enzyme immunoassay (EIA). It has been suggested that that the biological activity, as measured by binding to the Ah receptor, of the brominated and mixed bromo/chloro compounds is similar to their chlorinated analogues. PBDD/F, PCDD/F, and PBCDD/F laboratory standards exhibited activity ranging over three orders of magnitude. The highest relative potencies (REP) values relative to 2,3,7,8-TCDD were 2,3,7,8-TBDD (0.99 ± 0.07), 1,2,3,7,8-PeCDD (0.69) and 2-Br-3,7,8-TriCDD (0.72 ± 0.02). Cross-reactivities were calculated using EIA for several PBDDs/Fs and PBCDDs. 2,3,7,8-TBDD had 138 ± 34% cross-reactivity 16 when compared to 2,3,7,8-TCDD, followed by 2,3,7-TriBDD with 84 ± 36%.
