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Hepatotoxicity of Chlorpyrifos in Zebrafish Liver Cells by NMR-based Metabolomics
Citation:
Huang, W., Q. TENG, D. R. EKMAN, AND T. W. COLLETTE. Hepatotoxicity of Chlorpyrifos in Zebrafish Liver Cells by NMR-based Metabolomics. Presented at Metabolomics Society's 4th Annual International Conference, Boston, MA, September 02 - 06, 2008.
Impact/Purpose:
The objective of this research to develop metabolomic techniques and apply them to discover exposure biomarkers, characterize the important temporal aspects of these exposures, and define characteristics of exposures to which organisms can compensate.
Description:
For decades chlorpyrifos (CPS) has been one of the most widely used organophosphate insecticides for a variety of agricultural and public health applications. The extensive use of CPS inevitably results in exposure to a small number of the human population. It is believed that the chemical is metabolically oxidized to its oxygen analog, chlorpyrifos oxon (CPO), which can cause acute toxicity in humans by the inhibition of acetylcholinesterase in the central nervous systems. The metabolical oxidization of CPS to CPO primarily occurs in livers by multiple cytochrome P450 (CYP) enzyme isoforms. The aim of this study is to identify the metabolic changes in zebrafish (Danio rerio) liver cells (ZFL) caused by CPS and CPO at different dose levels and to investigate any differences in the cells response to the exposure of the two chemicals. This study is also a proof-of-concept for in-vitro metabolomics using fish cell culture as a model for testing chemical toxicity.