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Metabolic Changes Caused by Ethinylestradiol in Human Breast Cancer Cells as Observed by NMR Spectroscopy
Citation:
TENG, Q., W. Huang, D. R. EKMAN, AND T. W. COLLETTE. Metabolic Changes Caused by Ethinylestradiol in Human Breast Cancer Cells as Observed by NMR Spectroscopy. Presented at Metabolomics Society's 4th Annual International Conference, Boston, MA, September 02 - 06, 2008.
Impact/Purpose:
The objective of this research to develop metabolomic techniques and apply them to discover exposure biomarkers, characterize the important temporal aspects of these exposures, and define characteristics of exposures to which organisms can compensate.
Description:
To conduct cell culture based metabolomics, we have developed a novel sample preparation method using adherent mammalian cells, which is rapid, effective, and exhibits greater metabolite retention by approximately a factor of 50 compared to the conventional sample preparation method. We have applied this approach to study the metabolic changes caused by 17alpha-ethynylestradiol (EE2) in estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 human breast cancer cell lines using NMR spectroscopy. Details of sample preparation, NMR spectroscopy, metabolite identification, statistical analysis and intracellular metabolic changes will be presented.