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Toxicity Assessment of 17 alpha-ethinylestradiol by Cell-Culture Based NMR Metabolomics
Citation:
EKMAN, D. R., Q. TENG, W. Huang, AND T. W. COLLETTE. Toxicity Assessment of 17 alpha-ethinylestradiol by Cell-Culture Based NMR Metabolomics. Presented at Metabolomics Society's 4th Annual International Conference, Boston, MA, September 02 - 06, 2008.
Impact/Purpose:
The objective of this research to develop metabolomic techniques and apply them to discover exposure biomarkers, characterize the important temporal aspects of these exposures, and define characteristics of exposures to which organisms can compensate.
Description:
A zebrafish liver cell line (ZFL) established from adult zebrafish has been used in a variety of biological research, including toxicology, pharmacology, developmental biology and molecular genetics. The goal of this study is to develop an in vitro approach to identify the responsive changes in cellular metabolic profiles induced by the exposure of chemicals. The metabolites of the cellular extracts were profiled by 1D NOESY NMR experiments. Statistical analysis showed that the perturbational metabolic profiles in the ZFL cells exposed to EE2 have both dose- and time-dependences. The results demonstrated that cell culture based metabolomics using zebrafish liver cell line can potentially be used to screen chemicals for ecotoxicity. We are currently applying the in vitro metabolomics approach to assess toxicity of chemicals in human cell lines. Details of sample preparation, NMR spectroscopy, metabolite identification, statistical analysis and cellular metabolite profiling will be presented.