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Newer Analytical and Fractionation Approaches for Detecting Endocrine-active Chemicals in CAFOs
Citation:
DURHAN, E. J., V. S. WILSON, M. SEPULVEDA, D. MARTINOVIC, L. LEE, J. M. LAZORCHAK, C. R. LAMBRIGHT, J. CAVALLIN, L. BLAKE, AND G. T. ANKLEY. Newer Analytical and Fractionation Approaches for Detecting Endocrine-active Chemicals in CAFOs. Presented at SETAC Annual Meeting, Tampa, FL, November 16 - 20, 2008.
Impact/Purpose:
The overall goal is to directly link the measurement of endocrine active components with the estrogenic/androgenic effects observed in the effluents for the purpose of assessing potential ecological risk.
Description:
Several labs in ORD and academic partners from different US universities are involved in an integrated, collaborative effort to better assess the magnitude and extent of the impact of estrogenic and androgenic hormones in waste from concentrated animal feeding operations (CAFOs) and determine the impact of current CAFO waste management strategies on the fate and effects of hormones. This includes the development of robust in vitro and instrumental analytical techniques to detect active hormones, and biologically based fractionation approaches to identify and quantify specific compounds responsible for endocrine (androgenic and estrogenic) activity of complex CAFO discharges. This poster presents aspects of this collaborative research with an emphasis on the analytical and fractionation methods. We will provide examples in which HPLC-based fractionation linked to indications of biological activity (estrogenic and/or androgenic) in cell lines are used to simplify complex CAFO samples for subsequent investigative MS analyses. Our overall goal is to directly link the measurement of endocrine active components with the estrogenic/androgenic effects observed in the effluents for the purpose of assessing potential ecological risk.