You are here:
Identification of Coordinately Regulated Functional Modules in Thyroid Tissues from Rats Exposed to a Tumorigenic and a Non-Tumorigenic Conazole Fungicide Using Oncomine®
Citation:
HESTER, S. D. Identification of Coordinately Regulated Functional Modules in Thyroid Tissues from Rats Exposed to a Tumorigenic and a Non-Tumorigenic Conazole Fungicide Using Oncomine®. Presented at 11th International Meeting of the Microarray and Gene Expression Data Society, Riva del Garda, ITALY, September 01 - 04, 2008.
Impact/Purpose:
This research is an extension of ongoing research using genomics and bioinformatic approaches in the conazole project. Using data already generated in our lab, we will compare it to a large oncogenomic database containing cancer signatures, called Oncomine.
Description:
Conazoles are triazole- or imidazole-containing fungicides used in agriculture and medicine. Using transcriptomic analysis of rat thyroid tissues exposed to either tumorigenic or non-tumorigenic structurally related conazoles, we identified new findings on thyroid gene expression of gene sets associated with the tumorigenic exposure that are strongly linked to human thyroid neoplasia (Hester and Nesnow, TAAP, 227:357-69, 2008). Briefly, we suggested that PPARγ was the primary controlling nuclear receptor whose activation by the tumorigenic compound, triadimefon, correlates with the observed phenotypic changes in the thyroid gland by altering a series of genes involved in cell proliferation. It is likely that other functional modules (groups of genes) are involved. To identify these putative modules we sought to compare our results with a broad collection of cancer signatures. We focused our analytic efforts by utilizing a database and research tools provided in Oncomine 3.0: Genes, Pathways, and Networks in a Collection of 18,000 Cancer Gene Expression Profiles. The Oncomine initiative collects all published microarray data and provides a data pipeline consisting of 3 layers: data input, data analysis, and data visualization in concert with the Oncomine database. Anticipated results will enrich our understanding of underlying tumorigenic process associated with triadimefon exposure.